Coding
Cry7Ca1

Part:BBa_K3407001:Design

Designed by: Alicia Rodriguez Molina   Group: iGEM20_TUDelft   (2020-10-08)


Cry7Ca1 toxin from Bacillus thuringiensis strain BTH-13


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 634
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1334
    Illegal XhoI site found at 313
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Cry protein, Cry7Ca1 (GenBank accession no. EF486523) from the Bt strain BTH-13 is a three-domain Cry protein with a molecular weight of 129,196.50 kDa, whereas the activated toxin has a molecular weight of 68 kDa. This part contains the activated toxin. The gene was codon-optimized for expression in Escherichia coli using the GenSmartTM Codon Optimization Tool. The forbidden restriction enzymes sites are the following: BioBrick forbidden restriction sites (EcoRI, XbaI, SpeI, PstI) and Type IIS forbidden restriction sites (BsaI, SapI and Bpil).

Source

Synthetically synthesised.


References

Ordered List

  1. Jing, X., Yuan, Y., Wu, Y., Wu, D., Gong, P., & Gao, M. (2019). Crystal structure of Bacillus thuringiensis Cry7Ca1 toxin active against Locusta migratoria manilensis. Protein science : a publication of the Protein Society, 28(3), 609–619.